Frontiers of Science eTALK: Reprogramming T cells with CRISPR

Marson portrait, copyright UCSF
18:00
19:00

Alexander Marson gives a Frontiers of Science eTALK on CRISPR

Second speaker of autumn 2020 in Frontiers of Science eTALK seminar series is Associate Professor Alexander Marson from University of California San Francisco, US. He has been invited to give a zoom-talk “Reprogramming T Cells with CRISPR” on November 5th at 18:00. 

Registration is open until November 3rd

Student! Are you interested in CRISPR and would like to discuss science with Alexander Marson? 
Frontiers of Science students lunch is back!
You can share a cup of virtual coffee with him after the seminar, without senior scientists and bosses around. Register to the seminar and send an email to biocityturku@bioscience.fi

 

The goal of Marson's lab is to understand genetic circuits that control human immune cell function in health and disease. They employ genomic techniques to map epigenetic circuits that establish highly specialized cell identities. Marson's team have begun to identify autoimmunity risk variants that disrupt immune cell circuits, and how pathogenic circuits may be targeted with novel therapeutics. His lab has developed new tools for CRISPR genome engineering in primary human T cells and they are now pursuing a comprehensive strategy to test how coding and non-coding genetic variation controls functional programs in the immune system. Genome engineered human T cells hold great potential for the next generation of cell-based therapies for cancer, autoimmunity, and infectious diseases.

 

Selected publications

Roth TL, Li PJ, Blaeschke F, Nies JF, Apathy R, Mowery C, Yu R, Nguyen MLT, Lee Y, Truong A, Hiatt J, Wu D, Nguyen DN, Goodman D, Bluestone JA, Ye CJ, Roybal K, Shifrut E, Marson A. 2020. Pooled Knockin Targeting for Genome Engineering of Cellular Immunotherapies. Cell. 181: 728-744.e21.
https://dx.doi.org/10.1016/j.cell.2020.03.039

Nguyen DN, Roth TL, Li PJ, Chen PA, Apathy R, Mamedov MR, Vo LT, Tobin VR, Goodman D, Shifrut E, Bluestone JA, Puck JM, Szoka FC, Marson A. 2020. Polymer-stabilized Cas9 nanoparticles and modified repair templates increase genome editing efficiency. Nat Biotechnol. 38: 44-49
https://dx.doi.org/10.1038/s41587-019-0325-6

Shifrut E, Carnevale J, Tobin V, Roth TL, Woo JM, Bui CT, Li PJ, Diolaiti ME, Ashworth A, Marson A. 2018. Genome-wide CRISPR Screens in Primary Human T Cells Reveal Key Regulators of Immune Function. Cell. 175: 1958-1971.e15
https://dx.doi.org/10.1016/j.cell.2018.10.024

Roth TL, Puig-Saus C, Yu R, Shifrut E, Carnevale J, Li PJ, Hiatt J, Saco J, Krystofinski P, Li H, Tobin V, Nguyen DN, Lee MR, Putnam AL, Ferris AL, Chen JW, Schickel JN, Pellerin L, Carmody D, Alkorta-Aranburu G, Del Gaudio D, Matsumoto H, Morell M, Mao Y, Cho M, Quadros RM, Gurumurthy CB, Smith B, Haugwitz M, Hughes SH, Weissman JS, Schumann K, Esensten JH, May AP, Ashworth A, Kupfer GM, Greeley SAW, Bacchetta R, Meffre E, Roncarolo MG, Romberg N, Herold KC, Ribas A, Leonetti MD, Marson A. 2018. Reprogramming human T cell function and specificity with non-viral genome targeting. Nature 559: 405-409
https://dx.doi.org/10.1038/s41586-018-0326-5

Schumann K, Lin S, Boyer E, Simeonov DR, Subramaniam M, Gate RE, Haliburton GE, Ye CJ, Bluestone JA, Doudna JA, Marson A. 2015. Generation of knock-in primary human T cells using Cas9 ribonucleoproteins. Proc Natl Acad Sci U S A 112: 10437-42
https://dx.doi.org/10.1073/pnas.1512503112

 

General guidelines regarding the Frontiers of Science eTALKs

  • Registration is obligatory and links to register to all Frontiers of Science Autumn 2020 seminars are available in BioCity Turku webpage (https://biocityturku.fi/biocityevents/frontiers-of-science-seminars/programme-autumn-2020/) and in our news feed.
  • Registration closes two days before the event and link to join zoom will be sent to the participants one day in advance.
  • Microphones and cameras need to be turned off during the presentation, but the audience is welcome to ask questions after the talk, preferably with video turned on to make the discussion as interactive as possible.
  • If you are a student and need a certificate for your attendance, please fill in your student number when registering to the events. After the seminar season is over, please contact BioCity Turku coordinator biocityturku@bioscience.fi.

If you have any questions regarding our Frontiers of Science eTALK seminar series, please don’t hesitate to contact BioCity Turku coordinator biocityturku@bioscience.fi